5. L-broth 或φb-broth可替代SOC培养基。此时,转化效率可能会降低。
|
·L-broth :
|
Ingredient
|
per liter water
|
|
|
Tryptone
|
10 g
|
|
|
Yeast extract
|
5 g
|
|
|
NaCl
|
5 g
|
|
用1 N NaOH调整pH到7.5并高压灭菌。
|
·φ b-broth:
|
Ingredient
|
per liter water
|
|
|
Tryptone
|
20 g
|
|
|
Yeast extract
|
5 g
|
|
|
MgSO4·7H2O
|
5 g
|
|
用1 N KOH调整pH到7.5并高压灭菌。
|
|
6. 稀释时使用SOC培养基。
|
7. 建议在选择培养基中加入 Chloramphenicol (30 μg/ml) 以保证F' 质粒稳定。
|
8. YT soft agar:
|
Ingredient
|
per 100 ml water
|
|
|
Tryptone
|
0.8 g
|
|
|
Yeast extract
|
0.5 g
|
|
|
NaCl
|
0.5 g
|
|
用1 N NaOH调整pH到7.6,加入agar至浓度为0.6%,并高压灭菌。
|
9. YT-plate:
|
Ingredient
|
per liter water
|
|
|
Tryptone
|
8 g
|
|
|
Yeast extract
|
5 g
|
|
|
NaCl
|
5 g
|
|
用1 N NaOH调整pH到7.5,加入agar至浓度为1.5%,并高压灭菌。
|
|