Antibodies to Neural Progenitor Cells: Bf1, Crx, Emx1, Irx3,L7/Pcp2, Otp, Otx2 and Rx
神经前体细胞（Neural progenitor cells）表达特异性的蛋白标志物，因此，这些标志物的抗体被应用于干细胞分化过程的研究，比如胚胎发育和器官发育。在再生医学研究中，这些抗体被用做干细胞神经分化的标记物。在免疫组织化学中，这些抗体用于组织辨别和Western blotting。
Antibody to
|
Cell type detected
|
Antigen
|
Species
|
Specificity
|
Code No.
|
Bf1
|
Cerebral neural
progenitor cells in the telencephalon
|
C-terminal peptide of
human/mouse Bf1
conjugated with bovine thyroglobulin
|
Human
Mouse
|
C-terminal region of
human and mouse Bf1
|
M227
|
Crx
|
Retinal photoreceptor
cells(cone and rod
cells) during embryonic development
|
KLH-conjugated peptide
fragments of human Crx
|
Human
|
Human Crx
|
M231
|
Emx1
|
Cerebral cortex
neurons during
embryonic development
|
KLH-conjugated peptide
of mouse Emx1
|
Mouse
|
C-terminal region of
mouse Emx1
|
M196
|
Irx3
|
Neural plate progenitor
cells involved in caudal
nerve development
|
KLH-conjugated peptide
from the C-terminal
region of mouse Irx3
|
Mouse
|
Mouse Irx3
|
M230
|
L7/Pcp2
|
Purkinje progenitor
cells
|
KLH-conjugated peptide
from the C-terminal
region of L7/Pcp2
|
Mouse
|
C-terminal region of
mouse L7/Pcp2
|
M194
|
Human
|
C-terminal region of
mouse L7/Pcp2;
Reacts with
human & mouse L7/Pcp2
|
M202
|
Otp
|
Hindbrain and
hypothalamic neurons
during embryonic
development
|
KLH-conjugated peptide
from the C-terminal
region of mouse Otp
|
Mouse
|
C-terminal region of
mouse Otp
|
M195
|
Otx2
|
Retinal photoreceptor
cells during embryonic development
|
KLH-conjugated
peptide from the C-terminal
region of Otx2
|
Mouse
|
C-terminal region of
mouse Otx2
|
M198
|
Rx
|
Retinal progenitor cells
|
KLH-conjugated peptide
fragments of Rx
|
Mouse
|
Mouse Rx
|
M228
|
Human
Mouse
|
Human & mouse Rx
|
M229
|
■ 产品应用
· Immunohistochemical staining on fixed tissue
· Western blotting under reducing conditions (Cat. # M196, M228, M229, M230, M231)
Immunohistochemical Staining with Polyclonal Antibody to Mouse Otx2 (Cat. No. M198) and DAPI. Tissue: Embryonic mouse, day 10 (E10), forebrain to midbrain. Fixation: 4% paraformaldehyde, 6 h. Permeabilization: 0.3% Triton X-100 in PBS. Blocking: 2% skim milk in PBS. Blue, DAPI stain; red, anti-Otx2 antibody.